Effects of Organic Carbon Source and Nitrogen Source on Biomass Accumulation of <i>Chlorella</i> sp.1067

Abstract

Abstract. Chlorella sp. has the ability of mixotrophs, and the optimization of its culture medium has significant effect on growth. This paper investigated the effects of organic carbon source and nitrogen source on the accumulated biomass of Chlorella sp. 1067. In order to select the optimal organic carbon source, nitrogen source, and concentration combination; then concluded a regression equation of the effect on dry weight. It aimed at analyzing the mixotrophic growth of Chlorella 1067, and laying the foundation for further study of the relationship between autotrophic and heterotrophic. The results showed that glucose was the best organic carbon source to promote the accumulated biomass efficiently while carbamide was the optimal nitrogen source. In addition, the interaction of organic carbon source and nitrogen source has been studied. The experiment investigated the combined effect of glucose and carbamide on dry weight, and concluded the regression equation. The optimal solution was 50 mmol/L glucose and 35.4 mmol/L carbamide. In this case, the maximum theoretical value of dry weight could be up to 0.726 g/L.