Production of Antifungal Compounds by Andalas Endophytic Bacteria (Morus macroura Miq.) Isolate ATB 10-6 at Fermentation Medium with Optimum Carbon and Organic Nitrogen Source

Abstract

Andalas (Morus macroura Miq.) is one of the endemic plants in West Sumatera, which is known to have bioactive compounds that have the potential as antifungal. One of the effective ways to produce bioactive compounds from the plants is by utilizing endophytic bacteria through a fermentation process. One of the factor that influence fermentation is the medium. The purpose of this study was to see the effect of carbon and organic nitrogen sources on the growth of the Andalas endophytic bacteria ATB 10-6 isolate and its ability to produce antifungal compounds. The carbon sources used were glucose, lactose, maltose, sucrose, and starch. The types of organic nitrogen sources used were yeast extract, malt extract, beef extract and peptone water. Bacterial growth was observed by measuring optical density (OD), while antifungal activity was carried out by diffusion method. The result showed that a starch and yeast extract was the best carbon and organic nitrogen for the Andalas endophytic bacteria growth. These two types of nutrients are also the best sources of organic carbon and nitrogen to produce antifungal compounds.