Effects Of Orange Juice And Vitamin C On The Expression Of Some Selected Genes ( Tnfr, Sod3, P53, Pgr ) In Sprague‐Dawley Rat Brain

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The comparative effects of Vitamin C and Nigerian local orange juice on the gene expression levels of Superoxide dismutase (Sod), Tumour necrosis Factor Receptor (Tnfr), p53 protein, and progesterone receptor (Pgr) genes were evaluated in rat neuronal tissues.5ml/kg bodyweight of extracted orange juice and 20mg/kg bodyweight Vit C were administered orally to Sprague Dawley rats, which were divided into control, Vit. C and orange groups. After the 7th day of administration, total RNA was extracted from the rat brains and normalized RNA was converted to cDNA. PCR Primer pairs were designed using Primer 3 and NCBI primer blast tool. Sybr green Real time PCR relative quantification was done.Orange juice extract contains 15.84 mg Vit C, 13.67g total sugar, 0.02 mg Sodium, 0.15 mg Iron, 11.48 mg Magnesium, 44.20 mg Calcium, 4.76 mg Potassium, 0.75 mg Zinc, and 22.9 mg Phosphorus. Relative quantification gene expression assays in the rats show a 400 fold change in Tnfr and 38 fold change in p53 gene in the orange administered group in comparison to the Vit C administered and control groups. Vit C caused a 420 fold change in the expression of Pgr gene in comparison with the orange administered group. Data show that orange juice administered at 5ml/kg body weight show higher levels of gene expressions of Tnfr and p53 genes, thereby indicating brain tumour preventing ability of oranges.