Abstract
Objective: To study the effects of single-nucleotide polymorphisms of the OPRM1 and ABCB1 genes on the analgesic effect and consumption of sufentanil after thoracoscopic-assisted radical resection of lung cancer.Methods: A total of 225 Chinese Han nationality patients undergoing thoracoscopic-assisted radical resection of lung cancer were enrolled in the present study. Among them, 132 were males (58.67%) and 93 (41.33%) were females having American Society of Anesthesiologists statuses classified as grades I or II. The rs1799971, rs563649 and rs1323040 genotypes of the OPRM1 gene and rs2032582, rs1045642 and rs1128503 genotypes of the ABCB1 gene were detected by Sanger sequencing. The state anxiety index and pressure pain threshold were assessed preoperatively. Sufentanil was administered intravenously to maintain anesthesia. The doses and side effects of sufentanil consumed 6 h (T1), 24 h (T2) and 48 h (T3) after surgery were recorded.Results: The sufentanil doses at T1, T2 and T3 were significantly higher in radical-operation lung cancer patients with mutant homozygous rs1799971 and rs1323040 loci in the OPRM1 gene and rs2032582 and rs1128503 loci in the ABCB1 gene. The doses of sufentanil consumed by mutant heterozygous lung cancer patients at T1, T2 and T3 were significantly higher than those consumed by patients without mutations, and the differences were statistically significant (P<0.05). There was no significant difference in sufentanil doses consumed by lung cancer patients with mutant homozygous, mutant heterozygous and wild-type rs563649 locus of the OPRM1 gene and rs1045642 locus of the ABCB1 gene at T1, T2 and T3 (P>0.05). There was no significant difference in the visual analog scale scores at T1, T2 and T3 for different genotypes of OPRM1 and ABCB1 genes in lung cancer patients (P>0.05). No significant difference was found between the adverse reactions of OPRM1 and ABCB1 genotypes in patients undergoing radical resection of lung cancer (P>0.05).Conclusion: The rs1799971 and rs1323040 polymorphisms of the OPRM1 gene and rs2032582 and rs1128503 polymorphisms of the ABCB1 gene are related to the analgesic effect and consumed dose of sufentanil in Chinese Han patients undergoing radical operation of lung cancer.
Highlights
Lung cancer is one of the most common malignant tumors in the world with high mortality and morbidity as well as the propensity to metastasize
The mutation of G allele to A allele was detected at rs2032582 genetic locus of the ATP-binding cassette sub-family B member 1 (ABCB1) gene, and the mutation of C allele to T allele was detected at rs1045642 and rs1128503 genetic loci of the ABCB1 gene
The present study focused on rs1799971, rs563649 and rs1323040 loci of the opioid receptor μ-1 (OPRM1) gene and rs2032582, rs1045642 and rs1128503 loci of the ABCB1 gene, and we found that the single-nucleotide polymorphism (SNP) at rs1799971 and rs1323040 loci of OPRM1 and rs2032582 and rs1128503 loci of ABCB1 were significantly correlated with sufentanil consumption in patients who underwent radical resection of lung cancer
Summary
Lung cancer is one of the most common malignant tumors in the world with high mortality and morbidity as well as the propensity to metastasize. Radical surgery combined with c 2019 The Author(s). The μ-opioid receptor, encoded by the human opioid receptor μ-1 gene (OPRM1), is the primary site of action for sufentanil. Recent studies found that single-nucleotide polymorphisms (SNPs) of OPRM1 significantly influence the analgesic effectiveness and side effects of sufentanil [5,6,7]. P-gp in humans is encoded by the gene of ATP-binding cassette sub-family B member 1 (ABCB1), with at least 38 SNPs. Several studies reported that ABCB1 genetic polymorphisms were correlated with the analgesic effectiveness and consumption of opioids [10]
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