Effects of Onosma dichroanthum Boiss. root extract on AGS human gastric cancer cell-line.

Abstract

One of the cancer-related deaths is gastric cancer in this area. Onosma dichroanthum Boiss. roots have been used as an antiseptic and anti-inflammatory for wound healing treatment. The aim of this study was to assess the in vitro cytotoxic and anticancer effects of O.dichroanthum Boiss. roots from the Golestan province of Iran. After identification of the taxonomical effect of O.dichroanthum Boiss., different concentrations of the hydroalcoholic root extract were used. Three different time periods (24, 48, and 72h) were used to treat AGS gastric cancer and L-929 normal fibroblasts cell lines. The evaluation of different concentrations of root extract was performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The 48h treatment affected cell survival, while the concentration of 64μg/mL was determined as IC50 concentrations at 48h incubation time. The 48h incubation time with 64μg/mL showed the best effectiveness on cancerous cell-line while being safe for normal cell-line. Our results show that O.dichroanthum Boiss. roots extract may have cytotoxic and safe effects on gastric cancer cell-line and normal cells in 48h treatment periods, respectively. The results indicated the O.dichroanthum Boiss. may be as an effective anticancer agent (gastric cancer).