EFFECTS OF NYCTANTHES ARBOR-TRITIS (HARSINGAR) PLANT EXTRACTS ON PROMASTIGOTE FORM OF LEISHMANIA DONOVANI IN IN-VITRO CONDITION

Abstract

Leishmaniasis is a group of disease caused by the members of kinetoplast protozoa of the genus leishmania comprising a group of unicellular organisms which are intracellular parasites in macrophage and other phagocytic cells of the reticuloendothelial system. Visceral leishmaniasis (VL) or Kala-azar a vector-borne parasitic disease caused by protozoan parasite (Chang, KP.; et.al, 1985). In India the traditional methods of treatment are based on the principles of ayurveda and naturopathy. Considering the aforesaid facts this project has been selected to search the plants for the treatment of Kala-azar. The plant products or plant extracts will certainly be cheaper than the allopathic drugs. Therefore, aim is to search a plant which is locally available and if not available, it can be purchased from the local market to provide cheaper and without side effect treatment to Kala-azar (Mishra P. 2008; W.H.O. Tropical diseases 2008. Effects of Nyctanthes arbor-tritis plant extracts on promastigote form of Leishmania donovani in in-vitro condition. Phytochemical screening of the plant extract showed the presence of alkaloid, avonoid, xed oil and fats, saponin, tannin and phenolic compounds. The selected plant obtained from Gaya, Bihar region & their ethanolic extracts will be prepared in the laboratory by Soxhlet apparatus in Ethanol solvent to use during experiment. The effects of plant extracts on the parasite will be studied by providing variable doses to the parasite. The plant extracts at varied concentration will be added into media containing the parasite in the ratio between extract & media determined during experimentation in the laboratory. After incubation at different time intervals of 24, 48 and 72 hours the effect of extracts on the parasite will be studied. The sample of parasite (promastigote of L. donovani) used for experiment (in-vitro) will be routinely culture in laboratory at 25± 2° with in different culture media after collecting parasites (sample) from the blood and bone marrow of patients of Kala-azar in the hospitals or research institutes. The plant extracts exhibit that with the increasing duration of time the efcacy of the plant extracts also increases signicantly in NA taken into the consideration