Abstract
The main objective of the present study was to induce callus form nodal explants of Phyllanthus niruri and, compared the quantification of Phyllanthin in callus and naturally propagated Phyllanthus niruri. For callus initiation, different sterile plant parts were transferred on MS medium supplemented with auxins either alone like 0.5-2.0 mg/l 2- 4 D or with varied concentration and combinations of cytokinins like 0.5 mg/l BAP and 0.5 mg/l KN. The petroleum ether, chloroform, ethyl acetate, ethanol and aqueous extracts of Phyllanthus niruri plant and callus were prepared and performed its phytochemical analysis. The total flavonoids and polyphenol were investigated to quantify the presence of polyphenol compounds in callus extract and plant extract. The quantification of Phyllanthin in plant extract and callus extract were performed by HPLC. Maximum callus (90.5%) induction from stem/leaf segments on MS medium supplemented with 1.0 mg/l 2,4-D with 0.5 mg/l BAP (6-benzylaminopurine) within 25 days which was fragile in morphology. Preliminary phytochemical revealed the presence of various secondary metabolites in different extract of plant. The concentrations of flavonoids polyphenol in ethanol extract of callus were higher compared to crude plant extract. Further Phyllanthin content in callus was found to be significantly increased in response to field grown plants.
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
