Abstract
Objectives: To investigate the potential role of GABAB Receptors (GABABRs) involved in the effect of Nourishing “Yin”-Removing “Fire” Chinese herb mixture (TCM) treatment on precocious puberty. Methods: Female Sprague-Dawley rats were randomly divided into four groups: normal (N), central precocious puberty (CPP) model (M), CPP fed with normal saline (S) and CPP fed with Nourishing “Yin”-Removing “Fire” Chinese herb mixture (TCM). Rats of postnatal day 5 were given a single subcutaneous injection of 240 μg danazol to establish CPP model rats. Rats of S and TCM groups were continuously administered with saline or nourishing “Yin”-removing “Fire” Chinese herb mixture since postnatal day 15. The expression of GABABRs was detected by means of real-time PCR and immunohistochemistry. Results: The expression of hypothalamic GnRH mRNA in M was significantly increased on the day of pre-puberty when compared with that of N (P B1 receptor (GABABR1) immunoreactive cells in the arcuate nucleus (ARN) was decreased in M when compared with that of N (P B2 receptor (GABABR2) immunoreactive cells in the ARN, MS and diagnonal band of broca (DBB) were decreased in M when compared with those of N (P < 0.05) and increased in TCM compared with those of M (P < 0.05) on the day of onset-puberty. At the same time, the mRNA expression of GABABR2 was significantly decreased in M compared with that of N (P < 0.01). Conclusions: These results indicated that the GABABRs might involve in the curative effect of Nourishing “Yin”-Removing “Fire” Chinese herbal mixture on CPP rats.
Highlights
Sexual precocity is one of the most popular endocrine disorders in children, with the incidence of 0.6% throughout the world and is ten times more common in girls than in boys
The mRNA expression of GABABR2 was significantly decreased in M compared with that of N (P < 0.01). These results indicated that the GABAB Receptors (GABABRs) might involve in the curative effect of Nourishing “Yin”-Removing “Fire” Chinese herbal mixture on central precocious puberty (CPP) rats
The present study aims to investigate whether GABABR may take a part in mediating the curative effects of nourishing “Yin” -removing “Fire” herbal mixture on female precocious rats and further explore the therapeutic mechanism of the herbal mixture in CPP
Summary
Sexual precocity is one of the most popular endocrine disorders in children, with the incidence of 0.6% throughout the world and is ten times more common in girls than in boys. GABA is one of the primary inhibitory neurotransmitters to regulate the secretion of GnRH via its receptors in the hypothalamus [2]. GABA receptors were widely distributed throughout the CNS and had two major subtypes: ionotrophic GABAA receptor (GABAAR) and metabotrophic GABAB receptor (GABABR). It has been proved that GABAAR might play a part in the curative effect of Chinese Herb Mixture on CPP through up- regulating its expression [3]. In many nuclei of hypothalamus there exist GABAB receptors, even can be seen in GnRH neurons [4]. The present study aims to investigate whether GABABR may take a part in mediating the curative effects of nourishing “Yin” -removing “Fire” herbal mixture on female precocious rats and further explore the therapeutic mechanism of the herbal mixture in CPP
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