Effects of nano-selenium and sodium selenite on SelP, GPx4 and SelW genes expression in testes of broiler breeder roosters

Abstract

Fertility is the main element affecting hatchability as an important indicator in breeder flocks’ performance. Roosters’ aging reduces their fertility. Using additives such as selenium helps to delay this reduction through antioxidant properties of Se. Replacing inorganic Se by the nano form in poultry diet improves fertility. The aim of this study was to assess effects of different amount of nano-Se on SelP, GPx4 and SelW gene expression in broiler breeder rooster’s testis. A total of 30 fifty-weeks-old broiler breeder males were randomly divided into five groups including a control group (fed basal diet) and four treatments containing basal diet plus 0.3 mg/kg sodium selenite (1), 0.15 mg/kg nano-Se (2) 0.3 mg/kg nano-Se (3) and 0.6 mg/kg nano-Se (4). The results indicated that mRNA expression levels of SelP, GPx4 and SelW genes increased significantly (p<0.05) after dietary supplementation of nano-Se compared to control and sodium selenite groups. In conclusion, dietary supplementation of nano-Se was more effective than sodium selenite on mRNA expression level of tested genes.