Effects of Nano-Selenium on Kindey Selenium Contents, Glutathione Peroxidase Activities and GPx-1 mRNA Expression in Mice

Abstract

The experiment was conducted to evaluate kindey selenium status, glutathione peroxidase (GPx) activity and GPx-1 expression in mice fed with nanoselenium. Sixty KM mice, female and male in half, were randomly divided into control, sodium selenite and nanoselenium groups. 0.5 milliliter of water, sodium selenite (2 μg Se/mL) and nanoselenium (2 μg Se/mL) were respectively supplemented to the three groups in oral (ig) every day. Whole experiment lasted for 28 days. Kindey selenium contents, GPx activities and GPx-1 mRNA expression were analyzed at experiment trrmination. The results showed that kindey selenium contents and GPx activities in nanoselenium group and sodium selenite group were very significantly higher than those in control group (P<0.01); kindey GPx activities in nanoselenium group were significantly higher than that in sodium selenite group (P<0.05). Kindey mRNA expression of GPx-1 was approx 166% higher in nanoselenium group and approx 157% higher in sodium selenite group than that in control group. Kindey mRNA expression of GPx-1 was approx 3.50 % higher in nanoselenium group than that in sodium selenite group. The results indicated that nanoselenium supplementation could significantly enhance kindey selenium contents, GPx activities and GPx-1 mRNA expression in mice, nanoselenium was more available than sodium selenite in increasing kindey selenium contents, GPx activities and GPx-1 mRNA expression in mice.