Abstract

Effects of n-butanol fraction of Gongronema latifolium leave extract on some liver function and histological parameters in CCl4-induced oxidative damage in Wistar albino rats were assessed. Fifty-four (54) Wistar albino rats were divided into treatment group and LD50 groups. Group A (normal control) was given feed and water, Group B (vehicle control) was injected with olive oil intraperitoneally, while the rest groups (C, D, E, F and G) were injected intraperitoneally with a single dose of CCl4 (148 mg/kg) as a 1:1 (v/v) solution in olive oil and all the animals were fasted for 36 hours. This was repeated once every week for a period of four (4) weeks. At the end of 28 days of treatment, liver marker enzymes studies showed that there was significant (p 0.05) difference of these liver marker enzymes and bilirubin levels between the normal control and induced treated groups. Antioxidant assay on the liver homogenate showed that there was significant (p 0.05) difference between the normal control and induced treated groups. These findings suggested that n-butanol fraction of methanolic leave extract of G. latifolium may have anti-hepatotoxic and antioxidative effects against CCl4-induced liver damage in rats.

Highlights

  • Gongronema latifolium (Asclepiadaceae), is a perennial climber forest leafy vegetable with woody hollow glaborous stems below and characterized by greenish yellow flowers [1]

  • Studies were carried out to investigate the phytochemical constituents of G. latifolium and to evaluate the antihepatotoxic activities of n-butanol fraction of methanolic leave extract of G. latifolium against oxidative damage induced by CCl4 in Wistar albino rats

  • The result showed that the packed cell volume (PCV) level of induced control group was significantly (P0.05) difference between the PCV level of the normal control animals and all the induced treated animals

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Summary

Introduction

Gongronema latifolium (Asclepiadaceae), is a perennial climber forest leafy vegetable with woody hollow glaborous stems below and characterized by greenish yellow flowers [1]. It is widespread in tropical Africa such as Senegal, Chad and DR Congo as well as grows in the forest of south eastern and western Nigeria where it is widely used for medicinal and nutritional purposes [2]. The qualitative phytochemicals screening of the methanolic leave extract of G. latifolium revealed the presence of glycosides, alkaloids, saponin, flavonoids, tannins, and the absence of free anthraquinone. Studies were carried out to investigate the phytochemical constituents of G. latifolium and to evaluate the antihepatotoxic activities of n-butanol fraction of methanolic leave extract of G. latifolium against oxidative damage induced by CCl4 in Wistar albino rats

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