Effects of microRNA-126 on proliferation of lung adenocarcinoma A549 cells

Abstract

Objective To explore the effect of microRNA-126 (miR-126) on the proliferation of lung adenocarcinoma A549 cells.Methods 549 cells in vitro were divided into 3 groups:group A treated only with liposomes,group B with miR-126 control,and group C with miR-126.The expression of miR-126 was detected by using Real-time quantitative polymerase chain reaction (Real-time PCR),and that of vascular endothelial growth factor (VEGF) protein by Western blotting,and methyl thiazol tetrazolium (MTr) assay was used to examine the growth of A549 cells.Results In group C,the expression level of miR-126 was increased to (0.786 ±0.132) as compared with group A (0.453 ±0.085) and group B (0.432 ±0.092) (P ＜0.01),and the VEGF protein expression was decreased to (0.312 ± 0.075) as compared with group A (0.633 ±0.172) and group B (0.597 ±0.095) (P ＜0.01).The growth of A549 cells in group C was inhibited significantly as compared with group A and group B (P ＜ 0.05).Conclusion MiR-126 transfection can significantly increase its expression in A549 cells,decrease the expression of VEGF protein,and suppress the cells growth. Key words: MicroRNA-126 ; Vascular endothelial growth factor; Proliferation ; Lung adenocarcinoma