Effects of methionine and vitamin B1 and sodium tanshinone II A sulfonate on tumor necrosis factor-α in plasma and malondialdehyde, superoxide dismutase in kidney and kidney function of rats with obstructive jaundice

Abstract

Objective To observe changes of kidney function,tumor necrosis factor-α (TNF-α)in plasma and renal superoxide dismutase (SOD) and malondialdehyde (MDA) of rats with obstructive jaundice (OJ) and effects of sodium tanshinone Ⅱ A sulfonate (STS) and methionine and vitamin B1 ( MVB1 ).Methods Sham-operation group was set up ( n =5 ),and OJ models were established by ligation of common bile duct in rats.OJ rats were randomly divided into experimental control group ( EC group,n =20),MVB1 group (n=20),STS group (n =20) and MVB1 and STS group (M-S group,n=20).From 1st to 13 day,the rate in MVB1 and STS groups were respectively injected with the same volume of isotonic sodium hloride,MVB1 and STS,respectively.The rats in M-S group received MVB1 and STS together.The expression levels of TNF-α,blood urea nitrogen (BUN) and creatinine (Cr) in plasma on the day 2,6,10 and 14 after operation,MDA and SOD in the kidney tissue on the day 14 were determined by using radioimmunoassay.The histopathological changes of kidney and mortality rate of rats were observed.Results In sham group,levels of TNF-α,BUN and Cr in plasma and MDA and SOD in the kidney tissue were (0.91 ±0.18) μg/L,(1.54±0.16) mmol/L,(44.23 ±4.73) μ mol/L,(4.54±0.46) nmol/ml,and ( 182.16 ± 12.10) U/ml respectively,In the OJ groups,there was significant chants inthe above indicators (P ＜ 0.01 ).As compared with EC group,levels of TNF-α,BUN,Cr and MDA were declined and those of SOD were increased in STS group ( P ＜ 0.05).There was significant changes in TNF-α,BUN and Cr in MVB1 grorp (P ＜0.05).There was statistically significnat difference in the levels of BUN,Cr,MDA and SOD between MVB1 and STS groups.Levels of TNF-α,BUN,Cr and MDA in M-S group were markedly lower than those in sham group,MVB1 and STS group (P ＜ 0.01 ),and levels of SOD were significantly higher.Under the light microscopy,histological examination showed hyperplasia of glomerular capillary endothelial cells,epithelial cells and mesangial cells,patchy necrosis,bile pigment deposition in renal tubules,and inflammatory cell infiltration.There were significant difference in histological changes between EC group with MVB1,STS,and M-S groups.Conclusion That levels of TNF-α in plasma and MDA in the kidney tissue were rapidly elevated and SOD decreased was one of the most important factors of damaging renal function in OJ rats.MVB1 and STS could restrain the increase of plasma TNF-α in OJ rats.STS has effects of protecting renal tissue in OJ rats from damaging by oxygen-derived free radicals.Combined use of MVB1 and STS exerts the syngistic effects,and the antiendotoxin effects were significantly enhanced. Key words: Obstructive jaundice; Methionine and vitamin B1 ; Sodium tanshinone Ⅱ A sulfonate ; Tumor necrosis factor-α; Superoxide dismutase