Effects of Methanolic Extracts of Anisopus mannii Leaves on Blood Glucose and Organ Weights of Wistar Rats with Alloxan Induced-Diabetes

Abstract

This study looked at the impact of Anisopus mannii methanolic leaf extract on Wistar rats’ organ weights and alloxan-induced diabetes. For this investigation, a total of 20 male Wistar (albino) rats weighing between 80 and 150 g were used. Four groups of rats were randomly assigned. 1: Normal control; 2: Negative control; 3: Positive control (treated alloxan induced-diabetic with 5 mg/kg metformin); 4: alloxan induced-diabetic treated with 200 mg/kg of methanolic A. mannii leaf extract. The findings of the study show there was a significant difference in the blood glucose levels between the diabetic rats treated with metformin and the diabetic rats treated with A. mannii methanolic leaves extract on days 7 and no significant difference in day 14. The fasting blood glucose level in the diabetic rats treated with A. mannii on day 7 reduced from 167.00±19.76 mg/dL to 88.08±11.78 mg/dL on day 14. the liver weights (3.69±0.21 g) of the diabetic rats treated with the extract were significantly lower than those of the diabetic rats treated with metformin (4.86±0.16 g), and it was also within the weight for normal control which were non-diabetic (3.93±0.29 g). The kidney weights of the diabetic rats treated with the extract (1.13±0.18 g) were significantly higher than those of the diabetic rats treated with metformin (0.96±0.03 g). This work unequivocally shows that, in rats given alloxan to produce diabetes, A. mannii extract was able to substantially lower fasting blood glucose levels and shield the kidneys and liver from harm in just two weeks..