Effects of mechanical ventilation method on inflammatory factors and pulmonary surfactant associated protein A of bronchoalveolar lavage fluid in newborn piglets with acute lung injury

Abstract

Objective To investigate the effects of 3 different ventilation methods, including conventional mechanical ventilation (CMV), high frequency oscillatory ventilation(HFOV) and partial liquid ventilation (PLV), on the changes of inflammatory factors and pulmonary surfactant associated protein A(SP-A) in bronchoalveolar lavage fluid(BALF) in newborn piglets with acute lung injury(ALI). Methods Twenty-four newborn piglets, no more than 3 days old, were enrolled.After ALI made with saline lavage(38 ℃, 35 mL/kg), newborn piglets were randomly assigned to 4 groups: control group (n=6, no ventilation), CMV group(n=6), HFOV group(n=6), and PLV group(n=6). Piglets were sacrificed after being ventilated for 24 h. Tumor necrosis factor-α(TNF-α), interleukin-8(IL-8), interleukin-1(IL-1) and SP-A in BALF were measured quantitatively by using enzyme-linked immunosorbent assay. Results In 3 groups using different ventilation methods, the population mean of TNF-α, IL-8, IL-1 and SP-A were statistically diffe-rent(all P=0.000). SP-A in PLV group and HFOV group were higher than that in CMV group (all P<0.05), while IL-8, IL-1 and TNF-α in PLV group were lower than those in CMV group (all P<0.05), IL-8 and TNF-α in PLV group were lower than those in HFOV group (all P<0.05), IL-8 and TNF-α in HFOV group were lower than those in CMV group (all P<0.05). Conclusions Pulmonary inflammatory reaction was different in 3 ventilation groups.Compared with CMV and HFOV, PLV attenuated inflammatory reaction, so it could increase the expression of SP-A and decrease the degradation of SP-A. Key words: Mechanical ventilation method; Bronchoalveolar lavage fluid; Inflammatory factor; Pulmonary surfactant associated protein A