Abstract
Objective To explore the effect of mechanical stimulation on the differentiation of neural stem cells (NSC) from embryos in vitro.Methods Neural stem cells were isolated from cerebra of mouse embryos with pregnant durations of 13.5 days.The harvested cells were cultured in serum-free media containing basic fibroblast growth factor (bFGF),and cyclic mechanical stretch was applied on neural stem cells by Flexercell 4000TM.The antigen on the cell surface was detected by using immunocytochemistry.The differentiation rate of the cells was observed under an inverted microscopy.Neural stem cells were identified by using immunocytochemistry.Results About 100％ of the differentiated cells with or without cyclic mechanical stretch showed positive nidogen.The percentage of Tubulin positive cells in stretch group and control group was (20.5 ± 3.4) ％ and ( 10.9 ± 4.7 ) ％.The differentiation rate of the cells in stretch group was increased significantly as compared with control group ( P ＜ 0.05 ).Conclusion Intermittent loading can affect the differentiation of neural stem cells from embryos in vitro.It can promote neural stem cells to differentiate into neurons. Key words: Neural stem cells; Cellular differentiation; Mechanical stimulation
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