Abstract
ABSTRACTIn order to investigate the effects of macrobenthos on the abundance and community composition of ammonia-oxidizing prokaryotes under different temperature conditions, laboratory microcosms containing two kinds of macrobenthos (Corbicula fluminea and Tubificid worms) were constructed. Real-time polymerase chain reaction (PCR) and clone libraries were applied to analyze the ammonia-oxidizing archaea (AOA) and bacteria (AOB) communities in the surface sediments. The lowest abundances of the archaeal and bacterial amoA gene were found in the samples cultured under 28 °C (archaeal amoA gene abundance, 2.71 × 106 copies/g dry sediment; bacterial amoA gene abundance, 1.17 × 107 copies/g dry sediment) of the C. fluminea group. However, there was no significant difference in terms of the abundance of archaeal amoA gene in all Tubificid worms treatment groups. Compared to the community composition of AOB, greater variations in the community composition of AOA were observed among the three different temperature groups.
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