Abstract
1. 1. Uptake of l-leucine, l-phenylalanine, l-proline and l-lysine into brush border membrane vesicles from rats fed either a medium-chain triglyceride (MCT) or a long-chain triglyceride (LCT) diet was studied under conditions of the presence or absence of a Na + gradient. 2. 2. From the results of initial rate, Na +-dependent transport in LCT feeding were lower than in feeding MCT. The Na +-independent transport did not vary in either group except for l-lysine uptake. 3. 3. For l-leucine, l-phenylalanine and l-proline in Na + dependence, kinetic analysis revealed 4–6-fold smaller V max values in LCT group than in MCT group. l-Lysine in Na +-independent transport was 10-fold lower in LCT group than in MCT group. The K m values were not affected by feeding the LCT or MCT diet. 4. 4. It is clear that amino acid transport is regulated by different types of dietary fat. We consider that the alteration of transport activity is attributable to the changes in number of membrane-bound transport carriers but not to their affinity.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Comparative Biochemistry and Physiology -- Part A: Physiology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
