Effects of ligustrazine on autophagy-related proteins after spinal cord ischemia-reperfusion injury

Abstract

Objective To investigate the effect of ligustrazine on autophagy-related proteins Beclin l, LC3 and P62 after spinal cord ischemia-reperfusion injury. Methods A total of 48 SD rats were randomly divided into sham operation group, model group, ligustrazine group and 3-MA group. The rats were intraperitoneally injected with ligustrazine injection 0.16 mg/kg in the Ligustrazine group, the rats were intraperitoneally injected with 3-methyladenine injection 0.015 mg/kg in the inhibitor group, and the rats were intraperitoneally injected with normal saline of equal volume in the sham operation group and model group. Spinal cord ischemia-reperfusion model was established in all groups except sham-operated group after administration. After molding behavioral scores were scored after 3 and 6 hours of ischemia, and the expression of Beclin l, LC3 and P62 was detected by immunohis-tochemistry. Results After 3 and 6 hours, compared with the model group, the behavioral score (3 h: 2.33 ± 0.58 vs. 0.67 ± 0.58, 6 h: 3.33 ± 0.58 vs. 1.33 ± 0.58) of the rats in ligustrazine group significantly increased (P<0.05). Compared with the model group, the expression of Beclinl (3 h: 348.00×104 ± 0.27×104vs. 659.00×104 ± 0.11×104; 6 h: 38.00×104 ± 0.19×104vs. 557.00×104 ± 0.26×104), LC3 (3 h: 357.00×104 ± 0.48×104vs. 686.00×104 ± 0.33×104; 6 h: 334.00×104 ± 0.51×104vs. 673.00×104 ± 0.22×104), P62 (3 h: 357.00×104 ± 0.48×104vs. 830.00×104 ± 0.48×104; 6 h: 315.00×104 ± 0.12×104vs. 591.00×104 ± 0.36×104) in ligustrazine group were significantly decreased (P<0.05). Conclusions The ligustrazine may regulate autophagy in two directions and protect nerve cells. Key words: Chuanxiongzine; Spinal cord ischemia; Reperfusion injury; Beclin-1; Rats