Abstract
Recently, light emitting diodes (LEDs) have been introduced as a potential physical factor for proliferation and differentiation of various stem cells. Among the mesenchymal stem cells human umbilical cord matrix-derived mesenchymal (hUCM) cells are easily propagated in the laboratory and their low immunogenicity make them more appropriate for regenerative medicine procedures. We aimed at this study to evaluate the effect of red and green light emitted from LED on the neural lineage differentiation of hUCM cells in the presence or absence of retinoic acid (RA). Harvested hUCM cells exhibited mesenchymal and stemness properties. Irradiation of these cells by green and red LED with or without RA pre-treatment successfully differentiated them into neural lineage when the morphology of the induced cells, gene expression pattern (nestin, β-tubulin III and Olig2) and protein synthesis (anti-nestin, anti-β-tubulin III, anti-GFAP and anti-O4 antibodies) was evaluated. These data point for the first time to the fact that LED irradiation and optogenetic technology may be applied for neural differentiation and neuronal repair in regenerative medicine.
Highlights
In the recent years, the physical factors such as light irradiation emitted from different sources including light emitting diodes (LEDs), have been introduced as a potential physical factor for proliferation and differentiation of various cell types[1,2,3]
We aimed at the present study to investigate the effect of red and green light irradiations on the neural lineage differentiation of Human umbilical cord matrix-derived mesenchymal (hUCM) cells in the presence or absence of Retinoic acid (RA) as a known chemical inducing factor
We could demonstrate that LED irradiation with or without RA pre-treatment enhances neural differentiation of hUCM cells, especially when neural-specific gene expression was considered
Summary
The physical factors such as light irradiation emitted from different sources including light emitting diodes (LEDs), have been introduced as a potential physical factor for proliferation and differentiation of various cell types[1,2,3]. HUCM cells exhibit reduced immunogenicity especially at lower passages[17] and can be induced to generate different type of cells, such as adipocytes, osteocytes[18], myocytes[19], hepatocytes[20], and neurons, oligodendrocytes and other glial cells[21,22,23] These cells have been successfully used for the treatment of acute myocardial infarction without elevation of immune responses[24]. We aimed at the present study to investigate the effect of red and green light irradiations (provided by appropriate LEDs) on the neural lineage differentiation of hUCM cells in the presence or absence of RA as a known chemical inducing factor
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