Abstract

Optimum growth of etiolated pea stem sections was obtained in a basal solution of 3-indoleacetic acid, cobalt chloride, and sucrose. The final length of pea stem sections was increased when sucrose was added 2, 4, or 6 hours after 3-indoleacetic acid and cobalt were added to the test solution. Benzimidazole at 100 to 200 p.p.m. and kinetin at 1 p.p.m. added to the basal solution inhibited growth of etiolated pea stem sections. Growth.of the pea stem sections was inhibited when they were treated for 4 hours with kinetin plus 3-indoleacetic acid, then transferred to the basal solution for 14 hours. There was no growth inhibition when pea stem sections were treated for 4 hours with kinetin alone, followed by 14 hours on the basal solution. Similar results with kinetin were obtained when barley coleoptiles were used and 2,4-dichlorophenoxyacetic acid was the growth-promoting substance. Ether and hot water extracts of pea stem tissue pretreated with kinetin and 3-indoleacetic acid did not appear to contain a growth inhibitor.

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