Effects of ketogenic and western diets on proliferation, vasculogenesis and oxidative stress in the liver

Abstract

In this study, it was aimed to investigate the effects of different diets on lipid peroxidation, antioxidants, proliferation and vasculogenesis in liver tissue. BALBC female rats (21) were divided as the group fed with standard chow (SC), the group fed with a ketogenic diet (KD) and the group fed with western diet (WD). The rats were fed with tap water and a rat diet specially prepared according to the determined diets for 4 weeks. Liver tissue oxidative damage, proliferation and vasculogenesis were evaluated using spectrophotometric and immunohistopathological methods. At the end of the experiment, it was found that the highest weight gain was in the WD group and the least weight gain was in the KD group. The mean body weight of the WD group was statistically significantly higher compared to the SC and KD groups (p<0.05). MDA levels were found to be lower in the KD group compared to the SC and WD groups. GSH and CAT levels were higher in the KD group compared to the SC and WD groups. In IHC evaluation results, it was seen that Ki-67 percentage in the KD group increased compared to the WD and SC groups (p = 0.000), VEGF was close to each other in all three groups and there was no significant difference in the comparisons between groups (p>0.05). These results revealed that ketogenic diet prevents tissue damage by decreasing lipid peroxidation in liver tissue as well as inducing cellular proliferation and vasculogenesis.