Abstract
After intraductal injection of prolactin in pseudopregnant rabbits, a small increase in lipoprotein lipase occurred within 24 h, followed by a consistent rise from 72 h onwards. Intraductal injection of actinomycin D prevented this increase in lipoprotein lipase, whether given 4 h before, with, or upto 48 h after prolaction. At 72 h, the increased lipoprotein lipase activity of the tissue was reduced to basal activity by actinomycin D. Cycloheximide also inhibited the increase in lipoprotein lipase activity caused by prolactin, but had a shorter duration of effect than actinomycin D. The half-life of lipoprotein lipase in mammary tissue treated 5 days earlier with prolactin was 2.2 h after actinomycin D injection, and 1.1 h after cycloheximide injection. It is concluded that the prolactin-induced increase in lipoprotein lipase in mammary tissue requires the continued production of a short-lived messenger, and that the enzyme is rapidly inactivated in tissue once its synthesis is blocked by cycloheximide.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
