Effects of intermittent and constant high glucose on oxidative stress of bovine retinal capillary pericytes

Abstract

Objective To compare the effects of intermittent and constant high glucose on oxidativestress of bovine retinal capillary pericytes (BRPs). Methods BRPs were cultured for 6 days under constant different concentration glucose ( 15 mmol/L,25 mmoL/L), intermittent high glucose at the concentration of (5.5-15 mmol/L,5.5-25mmol/L ) and control group(5.5 mmol/L). TBA method was used to detect the contents of MDA. Xanthine oxidase method was used to detect the SOD activities. Results The MDA content/SOD activity of BRPs in intermittent and constant high glucose were higher than those in the control group( P ＜ 0.05 ). The changes were correlated positively with the concentrations of glucose ( P ＜ 0.01 ) and the amplitude of fluctuation ( P ＜0.05 ). The MDA content/SOD activity of BRPs in intermittent high glucose (5.5-15 mmol/L) were higher than those in the constant high glucose ( 15 mmol/L), the MDA content/SOD activity of BRPs in constant high glucose(25 mmol/L)were higher than those in the intermittent high glucose (5.5-25 mmol/L). Conclusion In certain glucose concentration range,the intermittent high glucose has astronger action on oxidative stress of BRPs than constant high glucose. Beyond a certain concentration of glucose, the constant high glucose has a stronger action on oxidative stress of BRPs than intermittent high glucose. Key words: Diabetic retinopathy ; Intermittent high glucose ; Oxidative stress ;