Abstract
Simple SummaryThis study was based on the determination of post-thaw semen quality, oxidant–antioxidant status and DNA-damage status of frozen Mesopotamian catfish semen. To this end, the sperm was frozen in diluent containing different inositol concentrations (5, 10, 20 and 40 mg). Increasing levels of inositol linearly increased the spermatozoa motility rate and duration significantly (p < 0.05). High doses of inositol increased antioxidants and decreased oxidants. In addition, lower intracellular DNA damage and percentage of apoptotic spermatozoa occurred in high doses of inositol (p < 0.05).In this study, the effects of supplemented inositol on sperm extenders were examined on the spermatozoa motility rate and duration, total antioxidant and oxidant status, apoptotic spermatozoa and DNA damage, during the sperm post-thaw process of Mesopotamian Catfish (Silurus triostegus, H. 1843). The semen was frozen in diluents containing different inositol concentrations (5, 10, 20 and 40 mg). Increasing levels of inositol linearly improved the spermatozoa motility rate and duration significantly (p < 0.05). MDA and TOS were linearly decreased, however, TAS and GSH linearly increased (p < 0.05). The increasing inositol levels resulted in a linear and quadratic decrease in DNA damage in the comet assay, 8-hydroxydeoxyguanosine and the determined percentage of apoptotic spermatozoa (p < 0.05). These results suggest that there are many positive effects of the use of supplemental inositol on enhancing sperm cryopreservation efficiency in Silurus triostegus.
Highlights
Silurus triostegus (H. 1843) (Mesopotamian catfish) live only in the Euphrates–Tigris basins [1,2,3,4]
The motility rate and duration, apoptosis, DNA damage and total antioxidant and oxidant status of the spermatozoa were evaluated in the thawed semen from frozen
There were significant linear and quadratic trend levels of inositol for the spermatozoa motility rate and motility duration (p < 0.05), where the spermatozoa motility rate and motility duration increased with the inclusion of 40 mg and 20 mg levels of inositol, respectively
Summary
Silurus triostegus (H. 1843) (Mesopotamian catfish) live only in the Euphrates–Tigris basins [1,2,3,4]. Sperm freezing can be effective in protecting population dynamics against possible risks due to increased stress factors in the natural environments of living organisms, and it is an effective tool for protecting genetic resources [8,9,10]. It allows the more effective use of gamete control methods, such as hybridization in aquaculture, and studies on subjects, such as refreezing thawed sperm [10,11,12]. In order to develop the optimum sperm freezing medium, different factors such as diluents, cryoprotectants, freezing rates, equilibration and freezing and thawing methods need to be examined separately [10,13,14]
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