Effects of influences of recombinant IFNα2b on the level of expression of the CD16, CD66b, CD33, CD11b receptors of neutrophilic granulocytes of conditionally healthy children in the experiment in vitro

Abstract

The polyfunctionality of neutrophilic granulocytes (NG) is the result of the rich equipment of NGs by structures that respond subtly to changes in the extracellular environment and intracellular processes. At the present time there is a wave of publications that note the importance of the full participation of NG in the cascade of immunological reactions. Wherein the authors assign a key role to NG, both at the start-up and subsequent regulation and realization of the immune response. The existence of diff erent populations of NGs diff ering in the profi le of equipping with membrane antigens and exhibiting various functional possibilities is shown. The aim of this study was to study the eff ects of recombinant IFNα2b (rIFNα2b) on a non-transformed and experimentally transformed subpopulation CD16+CD66b+CD33+CD11b+NG in vitro of peripheral blood samples of conditionally healthy children. The expression level of the membrane NG receptors was determined by fl ow cytometry. The studied indicators of untransformed NG (control) and NG after incubation with rIFNα2b and N-formyl-methionyl-leucyl-phenylalanine (fMLP) were assessed. The immunoregulatory eff ect of rIFNα2b on the nontransformed phenotype CD16+CD66b+CD33+CD11b+NG and the immunomodulatory eff ects of rIFNα2b on the fMLP-transformed NG phenotype of peripheral blood of conditionally healthy children were established.