Abstract
The in vitro response of the peach cv. Redhaven and of the peach/almond hybrid rootstock 'GF677' to increasing concentrations of NaCl in the medium is reported and discussed. Shoots with a single axis (microcuttings) were conditioned for 15 days on B5 medium (Gamborg et al., 1968) supplemented with 4.44 jimol 6benzylaminopurine (BA) and 0.1 mol sucrose, transferred to jars with the same medium containing 0 (control), 20, 100, 200 meq/1 NaCl and placed in growth chamber at 25°C and 16-hr photoperiod. Parameters of plant growth and mineral contents were recorded after 15 days. The trial was then replicated using 45 cc glass test tubes with cellulose plugs. The same parameters as in the previous trial along with chlorophyll a and b and, at trial's end, the mineral contents of the culture media were recorded. Shoot weight and growth decreased as saline concentration increased; the ratio of chlorophyll a to b in 'Redhaven' dropped notably as saline rate increased from 20 to 200 meq/1, and marked correlations of the Na/K ratio to salt concentration in medium were detected in the two cultivars as well as the expected sodium increase in plant tissues. A drop in the uptake of manganese and an increase of copper in the tissues along with the rising NaCl concentration was also recorded.
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