Effects of hypophysectomy on the plasma ionic and osmotic balance in rainbow trout, Salmo gairdneri

Abstract

The effects of hypophysectomy on the plasma ionic and osmotic balance in juvenile rainbow trout ( Salmo gairdneri) kept in 1 3 sea water for various periods were studied. Hypophysectomy caused a decrease in the plasma levels of ionized calcium and chloride within a week after the operation. At 2–5 weeks after hypophysectomy, the plasma levels of ionized calcium and chloride were still significantly lower in hypophysectomized fish when compared to sham-operated controls. Hypophysectomy had no effects on the plasma levels of sodium, total calcium, total magnesium, inorganic phosphate, plasma proteins, or plasma osmolality. When 1 3 sea water adapted (1–4 weeks), hypophysectomized and sham-operated fish were exposed to fresh water for 2 weeks, the plasma levels of ionized calcium, chloride, sodium, and plasma osmolality were significantly lower in hypophysectomized fish when compared to sham-operated fish, while the plasma levels of total calcium, total magnesium, and proteins were higher. No effects were noted on the plasma levels of potassium and inorganic phosphate. The presented data indicate that the pituitary gland plays a significant role in the regulation of plasma calcium and chloride levels in S. gairdneri, while its role in the regulation of plasma magnesium and sodium is less clear. The pituitary gland seems to play a minimal role in the plasma regulation of inorganic phosphate and potassium.