Effects of hydrotalcite on healing quality of active gastric ulcer with Helicobacter pylori infection

Abstract

Objective To observe the effects of hydrotalcite on histological ulcer healing quality in patients with Helicobacter pylori (Hp) positive active gastric ulcer. Methods A total of 145 patients with Hp positive active gastric ulcer were divided into two groups. The control group was treated with esomeprazole, amoxicillin and furazolidone triple therapy. The treatment group was treated with above triple therapy and hydrotalcite. After four-week treatment, gastroendoscopy was repeated. The sections of gastric biopsy specimens were stained with hematoxylin-eosin (HE) staining, Van Gieson staining, Alcian blue-periodic acid stiff (AB-PAS) staining, streptavidin-perosidase (SP) immunohistochemistry staining and computer imaging analysis technology were applied to observe maturity type of mucosal structure at the margin of ulcer, the content of collagen and neutral mucus, and the changes of expression of epithelial growth factor receptor (EGFR) and basic fibrobast growth factor (bFGF) before and after treatment. Paired samples t test was performed for comparison before and after treatment. F test and Chi-square test were used for comparison between two groups. Results The percentage of maturity type of regenerated mucosal structure of treatment group was 62.9% (39/62), however that of control group was 40.6% (26/64), and the difference was statistically significant (χ2=13.09, P=0.03). Compared with those before treatment, the content of collagen in granulation tissue and neutral mucus in regenerated mucosa increased in both groups after treatment, and the increase was more significant in treatment group ((55.1±10.4)% and (45.8±7.1)%, respectively); and the differences were statistically significant (F=12.85 and 18.17, both P<0.01). Compared with those before treatment, the percentage of EGFR and bFGF positive cells of both two groups increased, and the increase was more significant in treatment group ((49.5±8.4)% and (48.8±9.4)%, respectively), and the differences were statistically significant (F=12.17 and 18.73, both P<0.01). Conclusion Hydrotalcite combined with anti-Hp triple therapy can improve the maturity degree of structure and function of regenerated mucosa at the margin of ulcer in patients with Hp positive active gastric ulcer and then improve the healing quality of ulcer. Key words: Stomach ulcer; Helicobacter pylori; Healing quality of ulcer; Hydrotalcite