Effects of hydrogenated fat-spray-coated β-carotene supplement on plasma β-carotene concentration and conception rate after embryo transfer in Hanwoo beef cows

Abstract

We hypothesised that hydrogenated fat (HF)-spray-coated β-carotene (βC) supplement could be used to increase plasma βC concentration and conception rates after embryo transfer (ET) in Hanwoo beef cows. In Experiment 1, 12 multiparous Hanwoo cows were fed one of four experimental diets in a triplicate 4 × 4 Latin square design for a 28-day period. Treatments included no βC addition (control), HF-uncoated βC (HFuβC), HF-spray-coated βC (HFβC), and HF-spray-coated βC and vitamin A (HFβCA). The cows under βC-supplemented treatments were fed 400 mg/day of βC, and a daily intake for vitamin A of HFβCA treatment was 30 000 IU/day as retinyl acetate. Blood was collected on days 0, 26, 27, and 28 to analyse βC and other metabolite concentrations. In Experiment 2, 199 Hanwoo cows with low fertility were randomly assigned to either control (n = 99) or HFβC treatments (n = 100) based on the results of Experiment 1. The oestrus of the cows was synchronised for ET. The HFβC group was fed from 4 weeks before to 4 weeks after ET with a daily intake of 400 mg βC. Pregnancy for conception rates was diagnosed on day 60 after ET, and blood was collected for βC concentrations on the day before ET. Supplementing βC resulted in a high plasma βC concentration (P < 0.001). Supplementing HFβC or HFβCA resulted in higher βC concentrations than HFuβC (P < 0.001); however, there was no difference between HFβC and HFβCA groups. Plasma retinol concentration was lower in the HFβCA treatment than in the control and HFβC groups (P < 0.05). Blood metabolites were unaffected by the treatments. The retinol:βC ratio was lower in the βC-supplemented treatments than in the controls, and was lower in HFβC and HFβCA than in HFuβC groups (P < 0.001). Plasma βC concentration was positively correlated with plasma high-density lipoprotein, low-density lipoprotein, and total cholesterol (P < 0.05). Plasma retinol concentration was negatively associated with plasma protein (P < 0.01), but positively associated with plasma creatinine (P < 0.001) and urea (P < 0.01). Supplementing HFβC to low-fertility cows resulted in higher plasma βC concentration (P < 0.001) and conception rates (P = 0.024) than those in the controls. In conclusion, HFβC had a better bioavailability than HFuβC, and an increase in conception rates by supplementing HFβC may be beneficial for producing more calves given the low pregnancy rates of bovine ET in Korea.