Abstract
The effects of H2S on yellowing and energy metabolism of broccoli florets treated with hydrogen sulfide (H2S) or DL-propargylglycine (PAG) were investigated after four days of storage at 20°C. Our study showed that H2S treatment enhanced endogenous H2S content by 28.19% and 49.78% in comparison to the control and PAG-treated group respectively. This result might be related to the increase of l-cysteine desulfhydrase (LCD) and d-cysteine desulfhydrase (DCD) activities. Meanwhile, H2S treatment can maintain chlorophyll content at 0.329gkg−1, whereas the control at 0.298gkg−1 and PAG-treated group at 0.275gkg−1. This led to an alleviation of the yellowing in broccoli florets. In addition, high endogenous H2S content also activated the key enzymes, involved in energy metabolism, including ATPases, cytochrome C oxidase (CCO), succinate dehydrogenase (SDH), glucokinase, fructokinase, glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH). As a result, significantly higher energy charge was observed in H2S-treated group (p<0.05). These results suggest that H2S can effectively inhibit the yellowing and maintain high energy charge, therefore prolong the shelf life of postharvest broccoli.
Published Version
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