Effects of guar gum supplementation in high-fat diets on fish growth, gut histology, intestinal oxidative stress, inflammation, and apoptosis in juvenile largemouth bass (Micropterus salmoides)

Abstract

The present study aimed to investigate the influence of guar gum supplementation in high-fat diets on the growth performance and intestinal oxidative stress, inflammation, and apoptosis of juvenile largemouth bass. Five isonitrogenous diets were prepared: a control diet (10% crude lipid, C), a high-fat diet (17% crude lipid, HF), and three high-fat diets supplemented with 0.3% guar gum (GG0.3), 1% guar gum (GG1), and 3% guar gum (GG3). Largemouth bass (3.1±0.2 g) were randomly assigned to fifteen tanks (30 fish/tank) and fed for 8 weeks. The results demonstrated that GG0.3 significantly increased specific growth rate (SGR) and increased feed conversion ratio (FCR) compared to HF (P < 0.05). For histology, high-fat diets containing guar gum significantly increased intestinal villus length, villus width, and perimeter ratio, compared with HF (P < 0.05). Compared with Control, HF significantly decreased reduced glutathione (GSH) contents and increased malondialdehyde (MDA) contents in the intestine (P < 0.05). Additionally, HF significantly increased the expression of interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and cysteine-aspartic proteases 9 (Caspase 9) in the intestine (P < 0.05). Compared to HF, GG0.3 significantly decreased MDA contents, increased GSH contents, and downregulated the expression of IL-1β, TNF-α, and Caspase 3 than diet HF in the intestine (P < 0.05). These results suggest that guar gum can alleviate the adverse effects of high-fat diets on growth and gut health in fish.