Analysis of bacterial community of Labidochromis caeruleus reared in RAS with various feeds

Abstract

The electric yellow cichlid (Labidochromis caeruleus), renowned for its exceptional ornamental value, enjoys widespread popularity among aquarium enthusiasts and holds significant economic value in the market. This study employed high-throughput sequencing technology to analyze the microorganisms in the circulating water of the electric yellow cichlid cultivated with six different types of feeds. Statistical results showed that the compound feed group exhibited the highest specific growth and mass gain rates. In contrast, the water flea and brine shrimp groups showed lower rankings with significant differences. 51,084 to 64,626 effective sequences were obtained and clustered into 127 to 848 operational taxonomic units (OTUs). The research findings indicate a remarkable bacterial diversity in the breeding environment of electric yellow cichlids with higher levels in the water flea and brine shrimp groups, and the lowest level in the compound feed group; the dominant bacterial phyla in all six experimental groups include Proteobacteria, Actinobacteria, Parcubacteria, and Cyanobacteria; Comparative analysis using the COGs database revealed significant differences (P<0.05) in the metabolic pathways of 24 functional microbial genes among the experimental groups.