Abstract
BackgroundPanax notoginseng (Burk.) F. H. Chen (P. notoginseng) is a traditional Chinese medicine that has been used therapeutically for cardiovascular diseases, inflammatory diseases and traumatic injuries as well as for external and internal bleeding due to injury. Ginsenoside Rb1, a crucial monomeric active constituent extracted from P. notoginseng, has attracted widespread attention because of its potential anti-inflammatory, bacteriostatic, and cell growth-promoting effects. In this study, the therapeutic effects of ginsenoside Rb1 on second-degree burn in rats and the potential underlying mechanisms were explored.MethodsA rat model of second-degree burn injury was established, and skin wound healing was monitored at different time points after ginsenoside Rb1 treatment. HE staining was performed to identify burn severity, and biological tissues were biopsied on days 0, 7, 14, and 24 after treatment. Skin wound healing at different time points was monitored by macroscopic observation. Furthermore, IHC, WB, and RT-PCR were utilized to determine the protein and mRNA expression levels of PDGF-BB, PDGFR-β, and FGF-2 in wound tissues after treatment.ResultsHE staining showed that after 24 days of ginsenoside Rb1 treatment, skin tissue morphology was significant improved. Macroscopic observation demonstrated that in ginsenoside Rb1-treated rats, the scab removal time and fur growth time were decreased, and the wound healing rate was increased. Collectively, the results of IHC, WB and RT-PCR showed that PDGF-BB, PDGFR-β, and FGF-2 expressions peaked earlier in ginsenoside Rb1-treated rats than in model rats, consistent with the macroscopic observations.ConclusionCollectively, these findings indicated that ginsenoside Rb1 promotes burn wound healing via a mechanism possibly associated with upregulation of FGF-2/PDGF-BB/PDGFR-β gene and protein expressions.
Highlights
A single blister was observed on the skin surface of each second-degree burn model rat
We found that the size of the burn wounds was increased in all experimental groups except the blank control group on day 3 after burn injury
Our study suggests that ginsenoside Rb1 intervention accelerates the peaking of Platelet-derived growth factor-BB (PDGF-BB), Platelet-derived growth factor receptor-β (PDGFR-β), and Fibroblast growth factor-2 (FGF-2) protein and mRNA expression, an event that is related to the promotion of wound healing and a decreased wound healing time
Summary
Ginsenoside Rb1, a crucial monomeric active constituent extracted from P. notoginseng, has attracted widespread attention because of its potential anti-inflammatory, bacteriostatic, and cell growth-promoting effects. The therapeutic effects of ginsenoside Rb1 on second-degree burn in rats and the potential underlying mechanisms were explored. Second-degree burns affect the epidermis and some parts of the dermis [2]. Second-degree burn wound healing is a complex multifactorial process. During the proliferation and maturation stage, fibroblasts and myofibroblasts generate collagen and extracellular matrix components and form a bridge between the wound edges. Fibroblasts and their associated growth factors play a key role through out the process of wound repair [9]. The expression levels of FGF-2 and PDGF-BB/PDGFR-β can accurately reflect the progression of wound healing, and these molecules are important targets for research on the effects of drugs on wound healing
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