Abstract
The aim of this research is to investigate effect of gamma irradiation on antioxidant properties and microbial contaminants in Boletus griseipureus. Samples were ground into powder and later subject to gamma irradiation at 2.5, 5, 7.5 and 10 kGy. The non-irradiated and irradiated samples were then analyzed for their antioxidant properties by techniques including 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP), total phenolic content, beta glucan content and total triterpenoids. In addition, the microbial contamination was also evaluated. In terms of antioxidant properties, results showed that gamma irradiation did not result in significant effects on FRAP, total phenolic content, beta glucan content, and total triterpenoids. However, at 10 kGy of gamma irradiation, DPPH radical scavenging activity notably increased (p≤0.05). In term of microbial contamination, irradiation dose of 5 kGy reduced the microorganism to meet standard level and irradiation dose of 10 kGy was found to decrease microorganism to complete absence. Therefore, this research suggested that a minimum irradiation dose of 5 kGy can serve as a useful treatment for preserve its quality and prevent microbial contamination.
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