Effects of fullerenol C60(OH)24 on physiological and compensatory erythropoiesis

Abstract

In this work, the effect of fullerenol on erythropoiesis was investigated in healthy rats and in rats that had suffered blood loss in the amount of 2% of body weight. Fullerenol C60(OH)24 was diluted in 2 mL of 0.9% sodium chloride solution and injected intraperitoneally in a dose of 0.1 or 0.2 mg/kg body weight. The counts of reticulocytes, erythrocytes, and serum erythropoietin levels were determined in 48 and 96 h, as were the numbers of erythroid cells and erythroblastic islands (EIs) of different maturity fractions in the bone marrow and the fraction of the red pulp in the spleen. In intact rats, the reconstruction of erythropoiesis was augmented 1.5-fold in response to 0.1 mg/kg fullerenol C60(OH)24 and 3.3-fold in response to 0.2 mg/kg fullerenol. Both doses increased the number of oxyphylic erythroblasts in the bone marrow twofold, and 0.2 mg/kg fullerenol increased the red pulp fraction in the spleen by 9%; at the same time, fullerenol did not affect the counts of reticulocytes and erythrocytes, or the serum erythropoietin level in the case of normal erythropoiesis. Following hemorrhage, exposure to a 0.2 mg/kg dose of fullerenol C60(OH)24 increased serum erythropoietin levels, delayed the maturation of erythroid cells in EIs, partially blocked the release of reticulocytes from the bone marrow, and promoted an increase in the relative spleen weight and the red pulp fraction in the spleen.