Abstract
An immediate allergic response is caused by a chemical mediator released from basophil and mast cells via a cell degranulation due to a reaction between an IgE antibody, bound with the IgE receptor on the cell membrane, and an antigen. Using cultured cells instead of conventional methods based on histamine release from mast cells, the assay method in the present study permits highly accurate mass screening, since it uses a well-established cell line of rat basophilic leukemia cells (RBL-2H3), and determines by colorimetry the enzyme activity of β-hexosaminidase as an index of chemical mediator release. Effects of food additives on the immediate allergic reaction were evaluated using a newly developed assay system. A total of 100 food additives were investigated for their effects on β-hexosaminidase release from RBL-2H3 cells. Most of the additives showed no action ; zinc sulfate, zinc gluconate, copper sulfate, eugenol, cinnamaldehyde, ammonium persulfate, thiabendazole, ethyl and propyl p-hydroxybenzoates and aluminium sulfates inhibited allergic reaction ; butylated hydroxy toluene and butylated hydroxy anisol promoted this reaction and was attributed to their action of injurying the cells.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
