Abstract
Abstract The influence of eluant flow rate on efficiency in reverse-phase and ion-exchange high performance liquid chromatography of proteins was studied. Both isocratic and gradient elution led to the same conclusions. The use of flow rates much lower than those generally employed was found to be important for the efficient separation of large and small proteins. Several eluant systems were also examined to determine the effects these had on the efficiency of the reverse-phase separation of proteins.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Published version (
Free)
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
