Abstract
Objective To investigate the pathogenesis of systemic lupus erythematosus (SLE) through detec-ting and analyzing the genotype and distribution of FcγRⅡ a receptor, and the levels of antineutrophilic cytoplas-mic antibody (ANCA) and circulating immune complex (CIC) in serum. Methods Sixty-nine SLE patientsand forty-eight healthy volunteers in Yunnan were recruited. FcγRⅡ a genotyping was performed by nest PCRwith allele-specific primers to identify the two allelic forms ( H131 and R131 ). The transcription of FcγRⅡ agene was examined by RT-PCR . The levels of ANCA and CIC were measured by ELISA . Results Comparedwith controls the percentage of SLE patients which ganotype was FcγRⅡ a-R131 homozygosity increased. Thegenotype distributions of SLE (45% with FcγRⅡ a-R/R131, 30% with H/R131 and 25% with H/H131)were different from controls (21% with FcγRⅡ a-R/R131,52% with H/R131 and 27% with H/H131). Mo-reover,the express of FcγRⅡ a-R/R131 mRNA was upregulated. The positive rates of ANCA(67.9% ) andCIC (86.1%) in FcγRⅡ aR/R131 positive SLE patients were different from FcγRⅡ a-H/R131 and FcRⅡa-H/H131 (ANCA:21.4% with H/R131 ; 10.7% with H/HI31 ; CIC: 18.4% with H/R131 ; 14. 0% with H/H131 ). Conclusions It suggested that homozygous R/R131 genotype of FcγRⅡ a can be a genetic risk factorfor SLE patients in Yunnan, In addition, FcγRⅡ a-R/R131 over-expression may lead to the high positive rateof ANCA and CIC in SLE patients. Key words: Systemic lupus erythematosus; Antineutrophilic cytoplasmic antibody; Fc receptor; Circulating im-mune complex
Published Version
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