Effects of EZH2-siRNA on proliferation and invasion of human esophageal squamous carcinoma cells

Abstract

Objective To investigate the influence of EZH2 gene down-regulation by RNA interference (RNAi) on the proliferation and invasion of human esophageal squamous carcinoma cell line Ecal09.Methods Two small interfering RNAs (siRNA) targeting EZH2 were transfected into Eca109 cells by using lipofectamine 2000.The proliferation capcity of Eca109 cells was determined by calculating the cell population doubling time,the cell cycle,the cell cloning and the percentage of apoptotic cells.The invasive ability of Eca109 cells was detemined by Transwell assay.Results In vitro experiment showed that Eca109 cells transfected with EZH2-siRNA had significantly longer cell population doubling time [ ( 26.7 ± 0.9) h vs ( 22.6 ± 0.8 ) h,P ＜ 0.01 ],lower percentage of cell cloning [ ( 39.1 ± 2.3 ) ％ vs(65.1±3.0)％,P＜0.01],higher cell apoptotic rate [(9.51±0.19)％ vs (1.11 ±0.08)％,P＜0.01 ],and lower cell invasion rate [ (35.0 ± 0.4) ％ vs (51.8 ± 0.7) ％,P ＜ 0.01 ] than control groups.However,there was no significant diference in the cell cycle at S phase between EZH-2-siRNA group and control group [(32.1 ±0.7)％ vs (31.9 ±0.4)％,P＞0.05].Conclusion EZH2 siRNA can reduce the proliferation and invasion of human esophageal squamous carcinoma cells,which suggests that EZH2 siRNA might be a potential gene therapertic target of human esophageal squamous carcinoma. Key words: Esophageal squamous carcinoma; EZH2; RNA interference; Proliferation; Invasion