Effects of extracellular concentration of calcium and intensity of stimulation on short-term plasticity in single inhibitory synapses between cultured hippocampal neurons

Abstract

We studied the characteristics of short-term plasticity in inhibitory synapses of cultured neurons of the rat hippocampus. In our experiments, we used techniques of voltage clamp in the whole-cell configuration and of local electrical stimulation (pairs of stimuli were applied to a single synaptic terminal of the GABA-ergic neuron under conditions of the blockade of spreading excitation). We demonstrated that an increase or a decrease in the extracellular concentration of calcium ions ([Ca2+]o) results in modifications of the pattern of this plasticity. Depression of the second postsynaptic response under conditions of normal [Ca2+]o was characterized by a paired-pulse ratio (PPR) equal, on average, to 0.78 ± 0.04 (n = 5). With a decrease in the [Ca2+]o to 0.5 mM, depression was changed to facilitation (PPR = 1.17 ± 0.08, n = 5), while with a rise in the [Ca2+]o to 5.0 mM, depression became more clearly pronounced (PPR = 0.48 ± 0.03, n = 5). Alterations of responses, which were determined by a decrease or an increase in the [Ca2+]o, differed significantly from those related to a decrease or an increase in the amplitude of presynaptic stimulation. Analysis of the parameters of the pairs of evoked inhibitory postsynaptic currents under conditions of various [Ca2+]o and different intensities of stimulation of the presynaptic terminal allows us to conclude that in these terminals calcium-dependent (and, probably, also voltage-dependent) mechanisms underlying control of short-term synaptic plasticity are present.