Effects of external Mn2+ activities on OsNRAMP5 expression level and Cd accumulation in indica rice

Abstract

Manganese (Mn) transporter OsNRAMP5 was widely reported to regulate cadmium (Cd) uptake in rice. However, the relationship between OsNRAMP5 expression level and Cd accumulation, impacts of external ion activities on OsNRAMP5 expression level and Cd accumulation are still unclear. Investigations of the relationship between OsNRAMP5 expression level and Cd accumulation in three indica rice genotypes were conducted under various external Mn2+ activities ranging from Mn deficiency to toxicity in EGTA-buffered nutrient solution. Results in this work indicated that OsNRAMP5 expression level in roots significantly up-regulated at Mn phytotoxicity compared to that at Mn deficiency, which may stimulate by the increasing uptake of Mn. Our work also demonstrated that root Cd concentration of all the tested rice decreased notably when external Mn2+ activity reached the level of toxicity. This may explain by the increasing competition between the excess Mn2+ and Cd2+ as well as the disorder of element absorption caused by root damage at Mn toxicity. Our work also revealed that the relationship between OsNRAMP5 expression level in roots and Cd accumulation in roots was insignificant for all the tested genotypes. Besides, OsNRAMP5 expression level in roots seemed more related to root Mn accumulation. The fact that function of OsNRAMP5 mainly focuses on Mn uptake, together with the fact that many transporter genes involved in Cd uptake might result in the insignificant correlation between OsNRAMP5 expression level and Cd accumulation in roots. At last, multi-level regulating and processing of the process from gene expression to protein translation might account for the inconsistent relationship between root OsNRAMP5 expression level and Cd accumulation in roots.