Abstract

Browning of subcutaneous white adipose tissue (WAT) is induced by stimulation of β3 adrenergic receptor agonist with cold exposure and exercise training. However, it is remained unknown whether browning of adipose tissue equally inducing between obesity and non-obesity. The purpose of the present study was to investigate the effects of exercise on browning of adipose tissue in obesity. Male Otsuka Long-Evans Tokushima fatty (OLETF) rats and Long-Evans Tokushima Otsuka (LETO) rats were used as models of obesity and non-obesity, respectively. The rats were divided into exercise and sedentary groups. The rats of exercise groups were placed in the cages with a running wheel for 12 h (20:00 to 8:00 h) daily from 19 to 21 weeks of age. Food and water were provided ad libitum. There were no significant differences in the amount of voluntary wheel running between animal strains. Although there were no significant differences in the expression level of uncoupling protein 1 (UCP1) of brown adipose tissue (BAT) between the exercise and sedentary groups of LETO rats, the expression levels were significantly higher in the exercise group than the sedentary group of OLETF rats. In the inguinal WAT, the expression levels of UCP1 were below the minimum detectable threshold among all groups in both strains. These results suggest differential sensitivity of activation of UCP1 in BAT between obesity and non-obesity. In addition, one of the reasons for undetectable exercise-induced UCP1 expression in the WAT could be due to the low intensity by voluntary wheel running. This study was approved by the Institutional Animal Care and Use Committee of Hiroshima University (A19-163) and conducted in accordance with the Hiroshima University Regulations for Animal Experimentation. All experiments complied with the National Institute of Health Guidelines for the Care and Use of Laboratory Animals.

Full Text
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