Abstract
Thirty-five Wistar rats weighing 200 - 250 mg were categorized into five treatment groups- the control, saline, cisplatin, pre-cisplatin and the post-cisplatin groups, each comprising of seven rats per group, to assess the effects of ethanolic leaf extract of Newbouldia laevis on cisplatin-induced testicular damage in Wistar rats. The control group received feed mash and water ad libitum, the saline group received equal volumes of physiological saline intraperitoneally with normal feeds and water. The pre-cisplatin group was treated with 100mg/kg body weight of ethanolic leaf extract of Newbouldia laevis for twelve weeks before being treated with 8mg/kg of cisplatin intraperitoneally for five days. Sacrifice was done after another twelve weeks. Cisplatin group received 8 mg/kg body weight of cisplastin for five days without any extract treatment, but had normal feeds and water for twelve weeks before sacrifice. The post-cisplatin group had 8mg/kg body weight of cisplatin for five days before being treated with 100mg/kg of ethanolic leaf extract of Newbouldia laevis for twelve weeks before sacrifice. The extract group received only the 100mg/kg of ethanolic leaf extract of Newbouldia laevis for twelve weeks without any other treatment before sacrifice. The effects of these treatments on body weight, testicular and epididymal weight, testicular volume, total and motile sperm count, sperm motility, percentage live:dead sperm, hormonal and haematological parameters as well effects on testicular and epididymal cytoarchitecture of the Wistar rats were assessed.There were significant (p<0.05) changes in body weight, testicular and epididymal weight, testicular volume, sperm, hormonal and haematological parameters in the treatments, compared to control. The pre-cisplatin treatment group showed significant (p<0.05) improvement in the induced changes caused by cisplatin, compared to the post-cisplatin group. The findings of this investigation confirms the protective effects of ethanolic leaf extract of Newbouldia laevis on cisplatin-induced gonadotoxicity in male Wistar rats.
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