Abstract
Pitoyo A, Anggarwulan E, Ariza I. 2017. Effects of encapsulation matrix on physical properties and germination viability of calcium-alginate encapsulated plbs of Grammatophyllum scriptum. Cell Bio Dev 1: 36-40. Grammatophyllum is a tropical epiphytic orchid commonly found in the moist areas of South-East Asia. Like most orchid species, the genus comprises species with a very small, micro-size seed mass and lacked endosperm.These plants commonly need an in vitro culture for mass propagation and seed germination. Upon germination, their undeveloped-embryos developed a globular mass cells, a protocorm. Occasionally, a structure similar to protocorm arises from tissue other than an embryo, thereby the term protocorm-like body (plb) were introduced. Here, we develop synthetic seed, hydrogel beads encapsulated G. scriptum plbs and possibility to germinate the seed and growth their embedded tissue.The objective of the research was to study the effects of the proportion of encapsulation matrix of G scriptum Bl.Synthetic seed made by complexation of sodium alginate with CaCl2 on physical properties and germination of protocorm-like bodies (plbs) embedded inside the hydrogel. The experiment was designed by a single factor-completely randomized design with the treatments of several combinations of Na-alginate/CaCl2ratios.The result showed that CaCl2 in all concentration except 25 mM formed spherical hydrogel beads in all level concentration of Na-alginate. Alginate in concentration 2% and 3% gave the optimum result represented by maximum germination index 100%.The formation of the new plbs varied among different explants even in single explant.The time of germination of each synthetic seeds was varied from 2 weeks until eight weeks after encapsulation.In conclusion, physical properties have no significant barrier for developing plbs to emergencies through penetration encapsulation matrix.
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