Effects of EHD2 interference on migration of esophageal squamous cell carcinoma

Mei Li,Xianting Huang,Xiaojing Yang,Jianguo Zhang,Huijie Wang,Guoliang Liu,Song He,Hui Shi,Junya Zhu,Qinglei Hang

doi:10.1007/s12032-012-0396-4

Abstract

C-Terminal EH domain-containing protein 2 (EHD2) of the EHD family is associated with plasma membrane. We investigated the expression of EHD2 in human esophageal squamous cell carcinoma (ESCC) and the EHD2 expression to study the therapeutic effect of chemotherapy drugs. Western blot and immunohistochemistry were used to measure the expression of EHD2 protein in ESCC and adjacent normal tissue in 98 patients. EHD2 protein level was reduced in ESCC tissues in comparison with adjacent normal tissues. Under-expression of EHD2 increased the motility property of ESCC cell TE1 in vitro by wound-healing assays and transwell migration assays, and it was concurrent with the decreased expression of epithelial marker E-cadherin. Under-expression of EHD2 in TE1 can cause resistance to cisplatin. Our results suggested that EHD2 low expression is involved in the pathogenesis of ESCC, and it might be a favorable independent poor prognostic parameter for ESCC.

Highlights

Esophageal squamous cell carcinoma (ESCC) is one of the most common clinical malignancies [1]
We investigated the expression of EH domain-containing protein 2 (EHD2) in human esophageal squamous cell carcinoma (ESCC) and the EHD2 expression to study the therapeutic effect of chemotherapy drugs
The results of 98 ESCC samples by immunohistochemical analyses are in Table 1, and the patients were divided into two groups: high EHD2 expressers and low EHD2 expressers

Summary

Introduction

Esophageal squamous cell carcinoma (ESCC) is one of the most common clinical malignancies [1]. Tumor metastasis plays an important role in the development of malignant tumors. Identifying functional metastasis genes and their molecular mechanisms underlying the metastatic process remains a top priority in the cancer research field. Studies have shown that the actin cytoskeleton plays an essential role in numerous aspects of cell biology such as cell adhesion, cell morphology, cytokinesis, and especially in migration [5]. EHD2 (C-Terminal EH domain-containing protein 2) is a plasma membraneassociated member of the EHD family, which regulates internalization and is related to actin cytoskeleton. EHD2 was linked to the plasma membrane through actin filaments [6]. EHD2 plays a role in membrane reorganization in response to nucleotide

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Results

Conclusion