Abstract
Differentially Expressed Proteins between Esophageal Squamous Cell Carcinoma and Adjacent Normal Esophageal Tissue Proteomics was employed to identify the differentially expressed proteins between esophageal squamous cell carcinoma (ESCC) and adjacent normal esophageal tissues. ESCC tissues and adjacent normal tissues were obtained from 10 patients with ESCC and the proteins were extracted and subjected to two-dimensional gel electrophoresis (2-DE). The differentially expressed proteins were identified after image analysis, and matrix assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) was used to confirm these proteins. Immunohistochemistry was then performed to detect the expressions of HSP27 and ANX1 in ESCC tissues and adjacent normal tissues. A total of 6 differentially expressed proteins were identified by peptide mass fingerprinting, among which SCCA1, KRT4 and ANX1 were down-regulated and TIM1, MnSOD and HSP27 up-regulated in the ESCC. Immunohistochemistry showed HSP27 was highly expressed in the ESCC which, however, had a low expression of ANX1. These findings were consistent with those in proteomics. There were differentially expressed proteins between ESCC and adjacent normal tissues. The investigation of differentially expressed proteins between ESCC and normal esophageal tissue may provide evidence for the molecular pathogenesis of ESCC.
Highlights
Esophageal squamous cell carcinoma (ESCC), the predominant histological subtype of esophageal cancer, is the fourth most common malignancy
esophageal squamous cell carcinoma (ESCC) tissues and adjacent normal tissues were obtained from 10 patients with ESCC and the proteins were extracted and subjected to two-dimensional gel electrophoresis (2-dimensional electrophoresis (DE))
The differentially expressed proteins were identified after image analysis, and matrix assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) was used to confirm these proteins
Summary
Esophageal squamous cell carcinoma (ESCC), the predominant histological subtype of esophageal cancer, is the fourth most common malignancy. 108 Xiong et al.: Differentially Expressed Proteins diagnosis [1]. The pathogenesis of ESCC has been found to be associated with some factors, the exact pathogenesis is still unknown [2]. The investigations on the differentially expressed proteins between cancer tissues and normal tissues may be beneficial for understanding the mechanisms underlying the pathogenesis and development of the cancers, and helpful to find cancerogenesis-related proteins and markers of cancers [4]. Two-dimensional electrophoresis (DE) with immobilized pH gradients (IPG), matrix assisted laser desorption ionisation timeof-flight mass spectrometry (MALDI-TOF-MS) and bioinformatics were employed to detect the differentially expressed proteins between normal tissues and ESCC tissues. We identified 6 differentially expressed proteins among which the expressions and locations of HSP27 and ANX1 in the cancer tissues and normal tissues were further determined by immunohistochemistry
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