Effects of direct interaction between fermented milk and rice novel dietary products with human enterocytes on cell growth and innate immunity peptides production

Abstract

s / Digestive and Liver Disease 46 (2014) e85–e127 e97 MS was made according to modified Criteria of American Hearth Association. Dietary habits were assessed by a dietician, using the one week alimentary diary. Results: We divided our population in: group A children with WHtR≥0.62 (193pts, age 9.4±2.4 yrs, BMI z-score 2.5±0.5), group B childrenwithWHTR<0.62 (204 pts, age 9.5±2.3 yrs, BMI z-score 1.9±0.3). Total Energy Intake was 1843±459kcal/day in group A and 1723.6±402kcal/day in group B (p=0.008). No difference was recorded between intake (%) of proteins, carbohydrates and fats, but Group A presented an intake by commercial foods greater than Group B (442±265.2 kcal/day vs 383±209.8 kcal/day, p=0.014). The prevalence rate of MS was 19.7% in group A and 4.4% in group B (p<0.001). Conclusions: Obese children at higher risk for MS eat larger quantities of commercial foods. A correct nutritional education is required to reduce cardiovascular risk. http://dx.doi.org/10.1016/j.dld.2014.07.084 EFFECTS OF DIRECT INTERACTION BETWEEN FERMENTED MILK AND RICE NOVEL DIETARY PRODUCTS WITH HUMAN ENTEROCYTES ON CELL GROWTH AND INNATE IMMUNITY PEPTIDES PRODUCTION Lorella Paparo1,∗, Rosita Aitoro2, Rita Nocerino2, Ylenia Maddalena2, Simona Caprio2, Francesco Amato2, Vincenza Pezzella2, Linda Cosenza2, Andrea Budelli 3, Francesca Fasano3, Carmen Di Scala2, Roberto Berni Canani2 1 Department of Translational Medical Science-Pediatric Section and European Laboratory for the Investigation of Food Induced Diseases, University of Naples “Federico I“, Naples, Italy 2 Department of Translational Medical Science-Pediatric Section and European Laboratory for the Investigation of Food Induced Diseases, University of Naples “Federico II”, Naples, Italy 3 RD LL-37, +7400%, vs control p< .05; fermented rice: HBD2, +620%; LL-37, +11150% vs control, p< .05). The maximal effective doseswere 115mg/ml for HBD2 and 1.15mg/ml for LL37. Themaximal effective dose on cell growth was 115mg/ml. The effect of fermented milk product on cell growth derived from fully thermostable component/s, the effects of fermented rice product on LL-37 synthesis and on cell growth derived from fully thermo-stable component/s and the effects of fermented rice product on HBD-2 synthesis derived at least in part from heat-stable component/s. Conclusions: Through a direct interaction with the enterocyte fermentedmilk and rice products are able to significantly stimulate cell growth and innate immunity peptides synthesis. These results open theway for a fast characterization of components responsible for the observed clinical effects. http://dx.doi.org/10.1016/j.dld.2014.07.085 DEVELOPMENT OF INTESTINAL FLORA IN BREASTFED NEWBORNS EXPOSED TO INTRAPARTUM ANTIBIOTIC PROPHYLAXIS Giacomo Tonti1,∗, Silvia Martini1, Luigi Corvaglia1,∗, Irene Aloisio2, Giuseppe Mazzola2, Diana Di Gioia2, Bruno Biavati2, Giacomo Faldella1 1 Azienda Ospedaliera Universitaria Sant’Orsola-Malpighi U.O. Neonatologia e Terapia Intensiva Neonatale, Universita di Bologna, Bologna, Italy 2 Dipartimento di Scienze Agrarie Area di Microbiologia Agroambientale, Universita di Bologna, Bologna, Italy Objective: Intrapartum antibiotic prophylaxis (IAP) currently represents the most effective strategy to prevent the early-onset Group B Streptococcus (GBS) neonatal disease. We aimed to investigate the effect of IAP on the development of gut microbiota in breastfed term infants. Methods: Term newborns, vaginally delivered and receiving ≥50% of own mother’s milk, were recruited on day 2 of life and allocated into two groups. Group 1: infants born to mothers with GBS-positive vaginal swab, IAP-exposed. Group 2: infants born to mothers with GBS-negative vaginal swab, unexposed to IAP. Two fecal samples for each infant were collected on day 7 and 30. Bifidobacterium spp., Bacteroides fragilis group and Lactobacillus spp. counts were detected by real-time PCR and compared between the two groups. Results: Seventy-six newborns were recruited (Group 1=41, Group 2=35). On day 7 (Table 1), the count of Bifidobacterium spp. was significantly lower in Group 1 compared to Group 2 (p<0.05), while no difference was detected on day 30 (Table 2). Consistently, Group1 showeda significant increase inBifidobacterium spp. count on day 30 compared to day 7 (p<0.05). No differences were detected on days 7 and 30 between Group 1 and 2 in the number of Bacteroides fragilis group and Lactobacillus spp. Conclusions: IAPaffects theearlyneonatalmicrobiotaby reducing Bifidobacterium spp. levels. Breastfeeding, which promotes