Abstract
In this study, the stem explants of Ludisia discolor were used as experimental materials to investigate the effects of 6-BA, NAA, Cu2+ and Ag+ on the induction of adventitious bud regeneration, and to analyze the most suitable culture conditions for stem explants regeneration. The results showed that when the medium was supplemented with 1.0 mg/L 6-BA, 0.75 mg/L NAA, 0.25 mg/L CuSO4, or 6.4 mg/L AgCl, the best regeneration effects would be gained, respectively. And the adventitious bud regeneration rate reached the maximum, which were 61.67%, 83.67%, 80.95% and 87.63%, respectively. The results of this study provided a theoretical basis for tissue culture and rapid propagation of L. discolor.
Highlights
This study aims to explore the effects of different factors on the regeneration of adventitious bud from stem explants of L. discolor, hoping to find the most suitable conditions for adventitious bud induction, and to provide reference for the rapid propagation of L. discolor
The stems of 0.5-1.0 cm of aseptic seedlings of L. discolor were used as explants
Our study showed that 6BA could promote cell division and differentiation at a low concentration, accelerating adventitious bud regeneration of stem explants of L. discolor
Summary
Rich.) is a medicinal plant belonging to Orchidaceae and the genus of Ludisia. It is often called "Goldenline Orchidaceae" [1]. Ludisia discolor is mainly distributed in China, Vietnam, Malaysia, Thailand and other places [2]. It can be found in northern and central Guangdong of China, Hong Kong, Hainan, Guangxi, and southeast of Yunnan, and mainly growing in the damp places under the slopes or gully evergreen broad-leaved forests at an altitude of 900 ~ 1300 m [3]. L. discolor has been listed as a second-class national protected plant in China [5]. If artificial cultivation methods such as plant tissue culture and rapid propagation are adopted, the shortage of wild L. discolor germplasm resources can be alleviated, and providing material basis for the cultivation of high-quality L. discolor varieties and the high valuable products, and providing scientific basis for the protection of wild germplasm resources of L. discolor [6]
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