Effects of different densities of mixed-cropping on 13C-photosynthate distribution and grain yield of maize.

Abstract

To explore the effects of different densities of mixed-cropping on 13C-photosynthate distribution and grain yield of maize, we measured photosynthetic characteristics, 13C-photosynthate distribution, dry matter accumulation, and grain yield under different planting densities (LD, 67500 plants·hm-2 and HD, 97500 plants·hm-2) under mixed-cropping (M, 1:1, 2:2) and monoculture of Zhengdan958 (ZD) and Denghai605 (DH). The results showed that with the increases of planting density, grain yield, 13C-photosynthate allocation to grain, dry matter accumulation, and leaf area index (LAI) increased, but the chlorophyll content and net photosynthetic rate decreased. No significant difference was observed between the monoculture and mixed-cropping at the density of 67500 plants·hm-2. However, at 97500 plants·hm-2, LAI, chlorophyll content, net photosynthetic rate and dry matter accumulation in mixed-cropping were higher than that in monoculture. Mixed-cropping promoted the transport of dry matter from the vegetative organs such as stem to the grain and the distribution of 13C-photosynthates to grain. Grain yield of summer maize significantly increased in mixed-cropping due to the increase of 1000-grain mass. Under high plant density, the mixed-cropping could enlarge photosynthetic area, maintain higher net photosynthetic rate, increase dry matter accumulation, improve the distribution of dry matter, promote the distribution of 13C-photosynthates to grains and thus increase the grain yield. Our results indicated that mixed-cropping could significantly increase the yield of close planting summer maize in Huang-Huai-Hai Plain.